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What was the most significant Stepping Stone to Civil War?

Missouri Compromise - 1820
Nat Turner's Rebellion- 1831
Wilmot Proviso - 1846-1850
Compromise of 1850
Fugitive Slave Act - 1850
The Kansas-Nebraska Act - 1854
The Dred Scott Case -1857
John Brown's Raid on Harper's Ferry - 1859
Lincoln's Election -1860
Battle at Fort Sumter - 1861
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